In this study, the complete chloroplast genome of O.sativa Temperate Japonica YunJing-24 was assembled using Illumina sequencing data.The complete chloroplast (cp) genome is 134,556 bp in length, including Baby a pair of invert repeats (IRA and IRB) regions of 20,797 bp, large single-copy (LSC) region of 80,615 bp, and small USB Extenders single-copy (SSC) region of 12,347 bp.A total of 129 genes were predicted in the genome, including 87 protein-coding genes, 37 tRNA genes, and 8 rRNA genes.Phylogenetic analysis confirmed the phylogenetic relationship between O.
sativa Temperate Japonica and other representative species.